Oil Red O (also known as Oil Red 24) is a synthetic dye that is commonly used in histology and cell biology to stain lipids, particularly fat deposits within cells. It is widely employed in various techniques to visualize and study lipid droplets in cells and tissues. Oil Red O is often used to assess the presence and distribution of lipids in biological samples and can be employed in conjunction with microscopy to observe lipid-rich structures or cells.
The procedure for using Oil Red O typically involves the following steps:
Fixation: The cells or tissues of interest are usually fixed with a suitable fixative (e.g., formalin) to preserve their structure.
Staining: After fixation, the samples are exposed to an Oil Red O solution, which is usually prepared in isopropanol or other suitable solvents. The dye binds to the lipid droplets.
Washing: Excess dye is washed away using a wash solution (e.g., 60% isopropanol), leaving behind only the Oil Red O bound to the lipids.